Healthy Cell Proliferation

Cell proliferation is the biological process of cells increasing in number over time through cell division mitosis in eukaryotic cells.

Healthy cell proliferation.

Aberrations in cell proliferation however can give rise to malignant transformation and cancer pathology. Although proliferation can readily be interpreted as viability absence of proliferation should not automatically be taken as a sign of cell death. Assays for cell proliferation may monitor the number of cells over time the number of cellular divisions metabolic activity or dna synthesis. In addition to proliferation or cytotoxicity apoptosis necrosis and cytostatic responses can all be evaluated using cell health assays.

Healthy human cells usually produce arginine via arginosuccinase synthase but in tumor cells due to the lack of these enzymes it becomes auxotrophic for arginine to survive and proliferate. These signals help normal cells to know when to reproduce and when to stop reproducing. Healthy cells actively proliferate whereas growth arrested senescent and dead or dying cells do not. The proliferation and self renewal of these cells depend on growth factors.

Normal cells reproduce properly. Cell proliferation refers to an increase in cell number due to cell division cytokinesis which occurs as the final step of the cell cycle. Viability is a measure of the number of living cells in a population whereas proliferation is a measure of cell division. Cell health below is a table to aid in reagent choice for cell health and proliferation labeling applications.

Red and white blood cell production is regulated with great precision in healthy humans and the production of leukocytes is rapidly increased during infection. Cells in culture are only a model system and are different than cells in their normal in vivo environment. Thus arginine offers a potential targeting therapy by limiting its level in tumor cells. Cell proliferation assays are a cornerstone of stem cell and cancer cell pathway analysis and drug testing for efficacy and safety.

Cell counting using viability dyes such as trypan blue or calcein am can provide both the rate of proliferation as well as the percentage of viable cells. Cellular nutrition systems empower you to enhance quality of life performance and healthspan by optimizing the building block of the body the cell. Except for sex cells all cells of the body reproduce by mitosis. It should be noted that not all viable cells divide.

Proliferation is an essential mechanism for normal tissue development regeneration and renewal. 3d cell cultures more closely mimic in vivo conditions but can present challenges for experimental design. Sex cells reproduce through a process called meiosis.